Composite

Part:BBa_K3619030:Design

Designed by: Isak Ingerholt   Group: iGEM20_Lund   (2020-10-15)


Enterokinase Fusion Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 553


Design Notes

His-tag is added for later possible protein purification and Ek will auto catalyze cleavage site. Codon optimized for expression in E. coli.

Source

Bovine Enterokinase.

References

1) https://www.sciencedirect.com/science/article/pii/S1046592802000128#FIG2

2) https://www.jbc.org/content/268/31/23311.long